Production of L-ribose from L-ribulose by a triple-site variant of mannose-6-phosphate isomerase from Geobacillus thermodenitrificans

Applied and Environmental Microbiology
Yu-Ri LimDeok-Kun Oh

Abstract

A triple-site variant (W17Q N90A L129F) of mannose-6-phosphate isomerase from Geobacillus thermodenitrificans was obtained by combining variants with residue substitutions at different positions after random and site-directed mutagenesis. The specific activity and catalytic efficiency (k(cat)/K(m)) for L-ribulose isomerization of this variant were 3.1- and 7.1-fold higher, respectively, than those of the wild-type enzyme at pH 7.0 and 70°C in the presence of 1 mM Co(2+). The triple-site variant produced 213 g/liter l-ribose from 300 g/liter L-ribulose for 60 min, with a volumetric productivity of 213 g liter(-1) h(-1), which was 4.5-fold higher than that of the wild-type enzyme. The k(cat)/K(m) and productivity of the triple-site variant were approximately 2-fold higher than those of the Thermus thermophilus R142N variant of mannose-6-phosphate isomerase, which exhibited the highest values previously reported.

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L DoongY C Cheng
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·M A Martí-RenomA Sali
Dec 26, 2006·Journal of Bacteriology·Eun-Ah ChoYu-Ryang Pyun
Jul 11, 2007·Applied Microbiology and Biotechnology·Cassandra De MuynckErick Vandamme
Jun 15, 2010·Journal of Bioscience and Bioengineering·Hyun-Jung KwonDeok-Kun Oh
Jul 10, 2010·Journal of Molecular Biology·Laura M van StaalduinenZongchao Jia
Apr 28, 2011·Applied Microbiology and Biotechnology·Yu Huan LiuGang Li

Citations

Oct 5, 2014·Applied Microbiology and Biotechnology·Zheng XuHong Xu
May 7, 2020·Applied Microbiology and Biotechnology·Jiajun ChenWanmeng Mu
Jul 4, 2018·Applied Microbiology and Biotechnology·Zheng FangWanmeng Mu
Feb 24, 2020·Applied Microbiology and Biotechnology·Ming ChenWanmeng Mu
Jun 26, 2020·The Journal of Microbiology·Soo-Jung KimSoo-Jin Yeom
Jun 4, 2020·Journal of Agricultural and Food Chemistry·Zijie LiXiao-Dong Gao
Jun 12, 2021·Food Research International·Shahid MahmoodWanmeng Mu

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.