PMID: 14051818Jul 1, 1963Paper

PRODUCTION OF LIPASE BY PSEUDOMONAS FRAGI IN A SYNTHETIC MEDIUM

Journal of Bacteriology
J A Alford, D A PIERCE

Abstract

Alford, John A. (U.S. Department of Agriculture, Beltsville, Md.) and David A. Pierce. Production of lipase by Pseudomonas fragi in a synthetic medium. J. Bacteriol. 86:24-29. 1963.-The growth and production of lipase by Pseudomonas fragi in 41 dehydrated media were determined. From a chemical analysis of the peptone medium which gave the maximal lipase production, numerous synthetic media were prepared. Good cell growth, but no lipase production, was obtained on a buffered ammonium sulfate-glucose medium. The addition of arginine, lysine, aspartic acid, and glutamic acid to this medium gave lipase production approaching that obtained in the peptone medium. Omission of the glucose had little effect on growth, but no lipase were produced. Sterilization of the glucose with either the amino acids or phosphate buffer had no effect on growth, but sharply reduced lipase production. Gas chromatographic analysis of the fatty acids liberated from lard, corn oil, and coconut oil indicated that medium composition had no effect on enzyme specificity. P. fluorescens produced very little lipase in any of the synthetic media examined.

Citations

Dec 1, 1965·Journal of the American Oil Chemists' Society·J A Alford, J L Smith
Jul 1, 1979·Biotechnology and Bioengineering·N KosaricD Gerson
Sep 1, 1974·The Journal of Applied Bacteriology·N Legakis, J Papavassiliou
Aug 9, 2016·Applied and Environmental Microbiology·Chia-Suei HungWendy J Crookes-Goodson
Sep 1, 1971·The Journal of Applied Bacteriology·E M Barnes, W Melton
Feb 23, 2010·Journal of Basic Microbiology·Satnam Singh Aulakh, Ranjana Prakash

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