PMID: 16512606Mar 4, 2006Paper

Production of miniantibodies to Agrobacterium tumefaciens VirE2 virulence protein by the method of phage display

Molekuliarnaia genetika, mikrobiologiia i virusologiia
Vladimir VelikovM I Chumakov

Abstract

The scFv miniantibodies to the recombinant protein VirE2 from Agrobacterium tumefaciens were obtained by the method of phage display. The miniantibodies were purified and tested using timmunodot method for binding to a recombinant protein from Escherichia coli and to the native protein VirE2 from A. tumefaciens. The functional activity of the miniantibodies was comparable to the activity of mouse polyclonal antibodies against the VirE2 protein.

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