Production of monoclonal antibodies and development of an ELISA for solamargine.

Cytotechnology
M IshiyamaH Shinohara

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml(-1) to 11.5 nmol ml(-1) of solamargine.

References

Jan 1, 1980·Journal of Immunological Methods·J W Goding
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka
Jan 1, 1981·Methods in Enzymology·G Galfrè, C Milstein

❮ Previous
Next ❯

Citations

Jul 27, 2010·Journal of Natural Medicines·Benyakarn PongkitwitoonWaraporn Putalun
Apr 8, 1998·Forensic Science International : Synergy·Y ShoyamaK Nishi
Nov 13, 2008·Cytotechnology·Y ShoyamaN Fukuda
May 25, 2004·The American Journal of Chinese Medicine·P LoungratanaY Shoyama
Oct 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yukihiro Shoyama
Oct 1, 1998·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H Tanaka, Y Shoyama
Apr 21, 2012·International Journal of Analytical Chemistry·Hiroyuki TanakaYukihiro Shoyama
Feb 3, 2006·Journal of Chromatography. a·W Oleszek, Z Bialy
Mar 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin YanHuihua Qu
Oct 3, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takuhiro Uto
Nov 23, 2017·Journal of Natural Medicines·Seiichi SakamotoSatoshi Morimoto
Jan 23, 1999·Journal of Natural Products·W PutalunY Shoyama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.