Production of monoclonal antibodies

Current Protocols in Immunology
W M YokoyamaDiane Miller

Abstract

This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).

Citations

May 20, 2011·BMC Immunology·Dongbum KimHyung-Joo Kwon
Apr 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tessa A M SteevelsLinde Meyaard
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pankaj BansalE Sally Ward
Sep 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Frank LennartzAnja T R Jensen
Aug 4, 2010·Journal of Virological Methods·Caroline H SeibertAguinaldo R Pinto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.