Production of monovalent antisera by induction of immunological tolerance for capsular typing of Streptococcus pneumoniae

FEMS Microbiology Letters
J Henrichsen, J B Robbins

Abstract

Hyperimmune and high-titered polyclonal pneumococcal antisera, specific for cross-reactive types within groups, were produced in adult rabbits. Purified capsular polysaccharide was injected intravenously into adult rabbits. One week later, these rabbits were given multiple intravenous injections of formalin-inactivated pneumococci of the cross-reactive type by an established method. Each of the resultant antisera were specific for the cross-reactive type indicating that the previous injection of the polysaccharide had induced epitope-specific tolerance. This method was successful for production of antisera against pneumococcal types 6A, 6B, 9N, 9V, 19F and 19A. Polyclonal rabbit pneumococcal antisera have some advantages over murine monoclonal antibodies for serologic studies and this method should be applicable for producing type-specific antibodies to cross-reactive polysaccharides of clinical interest. Further, this method is simpler and generally produces higher titered monovalent (factor) reagents than absorbed antisera.

References

Jan 1, 1978·Scandinavian Journal of Gastroenterology·P ElsassL Ranek
Jul 1, 1981·Canadian Journal of Biochemistry·M B PerryD Carlo
Oct 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·K G Rosell, H J Jennings
Dec 23, 1983·Carbohydrate Research·E Katzenellenbogen, H J Jennings

Citations

Aug 18, 1999·The American Journal of Medicine·J Henrichsen
Jan 5, 2010·The Journal of Veterinary Medical Science·Sachie SaitoMasumi Sato

Related Concepts

Metazoa
Antibodies, Bacterial
Antibody Specificity
Carbohydrate Sequence
Immune Sera
Natural Immunosuppression
Polysaccharides, Bacterial
Chinchilla Rabbits
Streptococcus pneumoniae
Bacteriocin Typing

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