Production of muconic acid in plants

Metabolic Engineering
Aymerick EudesDominique Loqué

Abstract

Muconic acid (MA) is a dicarboxylic acid used for the production of industrially relevant chemicals such as adipic acid, terephthalic acid, and caprolactam. Because the synthesis of these polymer precursors generates toxic intermediates by utilizing petroleum-derived chemicals and corrosive catalysts, the development of alternative strategies for the bio-based production of MA has garnered significant interest. Plants produce organic carbon skeletons by harvesting carbon dioxide and energy from the sun, and therefore represent advantageous hosts for engineered metabolic pathways towards the manufacturing of chemicals. In this work, we engineered Arabidopsis to demonstrate that plants can serve as green factories for the bio-manufacturing of MA. In particular, dual expression of plastid-targeted bacterial salicylate hydroxylase (NahG) and catechol 1,2-dioxygenase (CatA) resulted in the conversion of the endogenous salicylic acid (SA) pool into MA via catechol. Sequential increase of SA derived from the shikimate pathway was achieved by expressing plastid-targeted versions of bacterial salicylate synthase (Irp9) and feedback-resistant 3-deoxy-D-arabino-heptulosonate synthase (AroG). Introducing this SA over-producing strategy int...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 23, 2003·Current Opinion in Plant Biology·Jyoti Shah
Jul 20, 2006·Critical Reviews in Biochemistry and Molecular Biology·Frédéric H VaillancourtLindsay D Eltis
Jun 30, 2007·Phytochemistry·Alison M MorseJohn M Davis
Feb 23, 2010·Current Opinion in Plant Biology·Frederik Börnke, Inge Broer
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lina Gallego-GiraldoRichard A Dixon
Feb 4, 2012·The Arabidopsis Book·D'Maris Amick DempseyDaniel F Klessig
May 5, 2012·Annual Review of Plant Biology·Hiroshi Maeda, Natalia Dudareva
Nov 13, 2012·Plant Biotechnology Journal·Fan YangDominique Loqué
Apr 3, 2013·Plant Physiology·Tariq A Akhtar, Eran Pichersky
Mar 4, 2014·Annual Review of Plant Biology·Gemma FarréChangfu Zhu
Apr 23, 2014·Biotechnology Advances·Neng-Zhong XiePing Xu
Dec 2, 2014·Current Opinion in Biotechnology·Kristi D SnellStevens M Brumbley
Dec 3, 2014·Metabolic Engineering·Ling Yuan, Erich Grotewold
Jan 13, 2015·Molecular Plant·Joshua R Widhalm, Natalia Dudareva
Oct 27, 2015·Nature Communications·Yang ZhangCathie Martin
Apr 1, 2016·The Plant Journal : for Cell and Molecular Biology·Patrick M ShihDominique Loqué
Mar 16, 2017·Journal of the American Chemical Society·Robert W HaushalterJay D Keasling
Apr 4, 2017·Current Opinion in Biotechnology·Nicholas S Kruyer, Pamela Peralta-Yahya

❮ Previous
Next ❯

Citations

Sep 27, 2018·Experimental Biology and Medicine·Jenny C Mortimer
Oct 7, 2019·Biotechnology Progress·Xiang Li, Yi Zheng
Jul 18, 2018·Applied Microbiology and Biotechnology·Muhammad BilalXuehong Zhang
Apr 23, 2020·Biotechnology for Biofuels·Chien-Yuan Lin, Aymerick Eudes
May 14, 2020·Environmental Science and Pollution Research International·Aliaksandr KolbasMichel Mench

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.