PMID: 16514280Mar 4, 2006Paper

Production of nitric oxide by murine macrophages induced by lipophosphoglycan of Leishmania major

The Korean Journal of Parasitology
G KavoosiZahra Tavakoli

Abstract

Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of the key determinants of parasite infectivity and survival is the surface glycoconjugate lipophosphoglycan (LPG). In addition, LPG is shown to be useful as a transmission blocking vaccine. Since culture supernatant of parasite promastigotes is a good source of LPG, we made attempts to characterize functions of the culture supernatant, and membrane LPG isolated from metacyclic promastigotes of Leishmania major. The purification scheme included anion-exchange chromatography, hydrophobic interaction chromatography and cold methanol precipitation. The purity of supernatant LPG (sLPG) and membrane LPG (mLPG) was determined by SDS-PAGE and thin layer chromatography. The effect of mLPG and sLPG on nitric oxide (NO) production by murine macrophages cell line (J774.1A) was studied. Both sLPG and mLPG induced NO production in a dose dependent manner but sLPG induced significantly higher amount of NO than mLPG. Our results show that sLPG is able to promote NO production by murine macrophages.

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Citations

Nov 19, 2008·Antimicrobial Agents and Chemotherapy·Raquel F RodriguesMarilene M Canto-Cavalheiro
May 10, 2012·Journal of Parasitology Research·Maria Fátima HortaLeda Quercia Vieira
Jan 11, 2019·Journal of Clinical Periodontology·Radhakrishnan NagarajanJeffrey L Ebersole
Apr 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Georgeta Serban
Jun 16, 2010·Annals of Dermatology·Jong Ho LimHyung Ok Kim
Oct 31, 2017·Asian Pacific Journal of Tropical Medicine·Adil M AllahverdiyevZeynep Akdeste
May 17, 2011·Mutation Research·Larissa Ragozo Cardoso de OliveiraSueli Aparecida Calvi

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