Production of novel antioxidative phenolic amides through heterologous expression of the plant's chlorogenic acid biosynthesis genes in yeast.

Metabolic Engineering
Andrea MogliaJules Beekwilder

Abstract

Phenolic esters like chlorogenic acid play an important role in therapeutic properties of many plant extracts. We aimed to produce phenolic esters in baker's yeast, by expressing tobacco 4CL and globe artichoke HCT. Indeed yeast produced phenolic esters. However, the primary product was identified as N-(E)-p-coumaroyl-3-hydroxyanthranilic acid by NMR. This compound is an amide condensation product of p-coumaric acid, which was supplied to the yeast, with 3-hydroxyanthranilic acid, which was unexpectedly recruited from the yeast metabolism by the HCT enzyme. N-(E)-p-coumaroyl-3-hydroxyanthranilic acid has not been described before, and it shows structural similarity to avenanthramides, a group of inflammation-inhibiting compounds present in oat. When applied to mouse fibroblasts, N-(E)-p-coumaroyl-3-hydroxyanthranilic acid induced a reduction of intracellular reactive oxygen species, indicating a potential therapeutic value for this novel compound.

Citations

Dec 15, 2010·PloS One·Teun W J M van HerpenJules Beekwilder
Oct 26, 2010·Applied Microbiology and Biotechnology·Aymerick EudesDominique Loqué
Sep 20, 2015·Metabolic Engineering·Shuang Ping LiuGui Yang Shi
Jun 14, 2018·Natural Product Reports·Benjamin J KotopkaChristina D Smolke
Dec 6, 2011·FEMS Yeast Research·Michael S SiddiquiChristina D Smolke
Jul 6, 2017·Apoptosis : an International Journal on Programmed Cell Death·Yi-Chun WangWei Shuai
Nov 7, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marwa RoumaniRomain Larbat

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here