PMID: 2125821Dec 1, 1990Paper

Production of phosphatidylethanol by phospholipase D phosphatidyl transferase in intact or dispersed pancreatic islets: evidence for the in situ metabolism of phosphatidylethanol

Archives of Biochemistry and Biophysics
S A Metz, M Dunlop

Abstract

To determine if phospholipase D is present in intact adult islets, we took advantage of the fact that, in the presence of ethanol, this enzyme generates phosphatidylethanol via transphosphatidylation. Extracts of cells prelabeled with [14C]arachidonate, [14C]myristate, or [14C]stearate were analyzed via three TLC systems; the identify of phosphatidylethanol was further confirmed via incorporation of [14C]ethanol into the same phospholipid bands. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate stimulated phosphatidylethanol (to 603% of basal by 60 min) both in intact adult islets and in dispersed neonatal islet cells. A nonphorbol activator of protein kinase C (mezerein) also stimulated phospholipase D, whereas a phorbol which does not activate protein kinase C (4 alpha-phorbol-12,13-didecanoate) was virtually inactive. The effects of the active phorbol ester or of mezerein were reduced by the protein kinase C inhibitor H-7 and were virtually eliminated by prior down-regulation of that enzyme. In addition, a calcium-selective ionophore (ionomycin) or fluoroaluminate also activated the islet phospholipase D. When accumulation of phosphatidylethanol (labeled with any of three fatty acids) was induced by a preincubation in t...Continue Reading

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Citations

Oct 31, 1991·Biochemical and Biophysical Research Communications·R J KonradB A Wolf
Jun 1, 1994·Alcoholism, Clinical and Experimental Research·C LundqvistL Gustavsson
Dec 4, 2012·International Journal of Molecular Sciences·Guido VielSanto Davide Ferrara
Jul 16, 2014·PloS One·José P Ferraz-NogueiraJuan Llopis
Oct 19, 2016·IUBMB Life·Christoph HeierRobert Zimmermann
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Apr 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Mikael LehtihetAke Sjoholm
Aug 1, 1996·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·G RydzewskaA Gabryelewicz

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