Production of polyclonal antisera

Current Protocols in Immunology
H M Cooper, Y Paterson

Abstract

Although the advances offered by the development of monoclonal antibody techniques have revolutionized the specificity, uniformity, and quantity of antibodies, there remain many circumstances in which polyclonal antibodies are more desirable than monoclonal antibodies. Production of polyclonal antisera takes less time and effort than production of monoclonal antibodies, requires relatively simple and readily available equipment, and produces reagents that can be used for immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assays (ELISAs). The Basic Protocol and Alternate Protocol in this unit describe the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters. The Support Protocol presents a method for preparing serum from blood.

References

Jan 1, 1975·Transplantation Reviews·N R Klinman, J L Press
Aug 30, 1992·Journal of Immunological Methods·B BennettR L Hunter
Jan 1, 1984·Annual Review of Immunology·D C BenjaminE M Prager

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Citations

Aug 24, 2012·Journal of the American Chemical Society·Alexis Vallée-BélisleKevin W Plaxco
Nov 9, 2017·Journal of Molecular Recognition : JMR·John W Schrader, Gary R McLean

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