Production of polyclonal antisera

Current Protocols in Immunology
Helen M Cooper, Yvonne Patterson

Abstract

Much of modern biology and biochemistry relies on the availability of highly specific antibodies for use in such ubiquitous techniques as immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting. Thus, the generation of large quantities of specific antibodies directed to proteins or peptides of interest is essential to the success of both basic and applied research programs. In addition, with the advent of antibody-based proteomic strategies for profiling protein expression and post-translational modification, a requirement for timely production of specific antibodies has emerged. Polyclonal antibodies derived from animals immunized with purified proteins or peptides are particularly valuable for use in the laboratory. This unit provides protocols for the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters.

References

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Jan 1, 1984·Annual Review of Immunology·D C BenjaminE M Prager
Sep 5, 2006·Journal of Immunological Methods·Karin LarssonHenrik Wernérus
Nov 2, 2006·Current Drug Delivery·Ming Tao LiangIstvan Toth
Mar 6, 2007·Vaccine·J C Aguilar, E G Rodríguez

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Citations

Mar 7, 2009·BMC Medical Genomics·Toni WhistlerNancy G Klimas
Aug 10, 2021·Frontiers in Plant Science·Otto GeigerIsabel M López-Lara
Dec 11, 2021·Current Protocols·Edward F RosloniecDavid D Brand
Aug 1, 2009·Current Protocols in Toxicology·Wen ZouHuizhong Chen

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