Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli

Frontiers in Plant Science
Ghulam Kadir Ahmad ParveezZamzuri Ishak

Abstract

Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including polymerase chain reaction (PCR), Southern blot, and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile blue A staining analyses confirmed the synthesis of PHB in some of the plantlets.

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Citations

Jul 29, 2018·Applied Microbiology and Biotechnology·Saikat GantaitSandeep Kumar Verma
Oct 31, 2019·International Journal of Molecular Sciences·Rajesh YarraHongxing Cao
Apr 8, 2020·Frontiers in Plant Science·Khaled Michel HazzouriKhaled M A Amiri

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Methods Mentioned

BETA
transgenic
PCR
electrophoresis
Assay
PCR and

Software Mentioned

RQ
7000 System SDS

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