Production of prostaglandin e(2) and i(2) is coupled with cyclooxygenase-2 in human follicular dendritic cells.

Immune Network
Whajung ChoJongseon Choe

Abstract

Prostaglandins (PGs) play pathogenic and protective roles in inflammatory diseases. The novel concept of PGs as immune modulators is being documented by several investigators. By establishing an in vitro experimental model containing human follicular dendritic cell-like cells, HK cells, we reported that HK cells produce prostaglandin E(2) (PGE(2)) and prostaglandin I(2) (PGI(2)) and that these PGs regulate biological functions of T and B cells. To investigate the respective contribution of cyclooxygenase-1 (COX-1) and COX-2 to PGE(2) and PGI(2) production in HK cells, we performed siRNA technology to knock down COX enzymes and examined the effect on PG production. Both PGE(2) and PGI(2) productions were almost completely inhibited by the depletion of COX-2. In contrast, COX-1 knockdown did not significantly affect PG production induced by lipopolysaccharide (LPS). The current results suggest that mPGES-1 and PGIS are coupled with COX-2 but not with COX-1 in human follicular dendritic cell (FDC) and may help understand the potential effects of selective COX inhibitors on the humoral immunity.

References

Feb 28, 2002·Trends in Immunology·Sarah G HarrisRichard P Phipps
May 16, 2002·International Immunopharmacology·Bianca Rocca, Garret A FitzGerald
Mar 28, 2003·Biochemical and Biophysical Research Communications·In Yong LeeJongseon Choe
Nov 7, 2003·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·J Vane, R E Corin
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·In Yong LeeJongseon Choe
Sep 6, 2005·Biochemical and Biophysical Research Communications·Noriko UenoMakoto Murakami
Oct 6, 2005·Journal of Lipid Research·Subhashini ChandrasekharanBeverly H Koller
Apr 15, 2006·The Journal of Clinical Investigation·Yan ChengGarret A Fitzgerald
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·In Yong LeeJongseon Choe
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Feb 23, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jini KimJongseon Choe

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