PMID: 8599332Apr 1, 1996Paper

Production of proteins in the milk of transgenic livestock: problems, solutions, and successes

The American Journal of Clinical Nutrition
A Colman

Abstract

The milk of livestock can be modified dramatically by introducing foreign DNA into the germline. Exclusive expression of this DNA is ensured by the presence of regulatory sequences from mammary gland-specific genes. In sheep > 50% of the protein in milk can be encoded by a transgene and it appears that the foreign protein is additional to the normal complement of proteins. However, many technical hurdles (DNA configuration, low efficiency of transgenesis, and transgene stability) still prevent the routine use of this technology. In addition, such milk products have not yet received regulatory approval. These difficulties are not insurmountable. Transgenic methods can also be used to study the molecular basis of milk biogenesis. The effect on milk production in mice with all endogenous alpha-lactalbumin genes removed and in mice in which the murine genes were then replaced with human homologues is described. alpha-Lactalbumin, and consequently lactose, is essential for normal milk formation, and the human gene is expressed more efficiently than the murine gene.

Citations

Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·A W ChanR D Bremel
Aug 26, 1998·Annual Review of Nutrition·M B Roberfroid, N M Delzenne
Feb 7, 2003·Journal of Biosciences·Narinder Kaur, Anil K Gupta
Jan 28, 2003·Nature Biotechnology·Brigid BrophyGötz Laible
Aug 9, 2008·Biotechnology Letters·Sharon F C LisauskasElíbio L Rech
Jan 21, 2009·Reproduction, Fertility, and Development·Ian WilmutJane Taylor
Jun 15, 2006·Amino Acids·E KarnaukhovaB Golding
Jan 29, 2016·Transgenic Research·L R BertoliniJ D Murray
Jul 28, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A P F Flint, J A Woolliams
Feb 26, 2008·Journal of Veterinary Science·Sang Tae ShinYoung Won Lee
Aug 19, 2020·Preparative Biochemistry & Biotechnology·Guihua GongYoujia Hu
Oct 6, 1997·Journal of Dairy Science·C N Karatzas, J D Turner
Nov 13, 2004·The Journal of Nutritional Biochemistry·P A PrietoB Kelder

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