Production of recombinant Chikungunya virus envelope 2 protein in Escherichia coli

Applied Microbiology and Biotechnology
Nagesh K TripathiAmbuj Shrivastava

Abstract

Chikungunya, a mosquito-borne viral disease caused by Chikungunya virus (CHIKV), has drawn substantial attention after its reemergence causing massive outbreaks in tropical regions of Asia and Africa. The recombinant envelope 2 (rE2) protein of CHIKV is a potential diagnostic as well as vaccine candidate. Development of cost-effective cultivation media and appropriate culture conditions are generally favorable for large-scale production of recombinant proteins in Escherichia coli. The effects of medium composition and cultivation conditions on the production of recombinant Chikungunya virus E2 (rCHIKV E2) protein were investigated in shake flask culture as well as batch cultivation of Escherichia coli. Further, the fed-batch process was also carried out for high cell density cultivation of E. coli expressing rE2 protein. Expression of rCHIKV E2 protein in E. coli was induced with 1 mM isopropyl-beta-thiogalactoside (IPTG) at ~23 g dry cell weight (DCW) per liter of culture and yielded an insoluble protein aggregating to form inclusion bodies. The final DCW after fed-batch cultivation was ~35 g/l. The inclusion bodies were isolated, solubilized in 8 M urea and purified through affinity chromatography to give a final product yiel...Continue Reading

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Citations

May 18, 2016·The Korean Journal of Parasitology·Zhaoshou YangHo-Woo Nam
Aug 30, 2020·Viruses·Flávia Fonseca BagnoFlávio Guimarães da Fonseca
Jul 26, 2018·Virology Journal·Marcílio Jorge FumagalliLuiz Tadeu Moraes Figueiredo
Feb 11, 2021·Molecular Biotechnology·Yvonne Jing Mei LiewBoon Chin Tan

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