PMID: 9655244Jul 9, 1998Paper

Production of recombinant hydroxylated human type III collagen fragment in Saccharomyces cerevisiae

DNA and Cell Biology
P R VaughnJerome A Werkmeister

Abstract

A recombinant hydroxylated fragment of human type III collagen has been produced in Saccharomyces cerevisiae by coordinated coexpression of a collagen gene fragment together with both the alpha- and beta-subunit genes for prolyl-4-hydroxylase (EC 1.14.11.2). The collagen fragment consisted of 255 residues of the helical domain and the complete C-telopeptide and C-propeptide domains. It was inserted under the control of the ethanol-inducible ADH2 promoter in a multicopy, TRP1-selectable, yeast expression vector, YEpFlag1. The prolyihydroxylase subunit genes were cloned on either side of a bidirectional galactose-inducible promoter in a low-copy minichromosome yeast expression vector, pYEUra3, which is URA3 selectable. Coordinated expression of the three different gene products after cotransformation into S. cerevisiae was detected by immunoblotting. Amino acid analysis of an immunoreactive collagen fraction demonstrated the presence of hydroxyproline, while the presence of a triple-helical domain in the collagen fragment was demonstrated by its resistance to pepsin proteolysis.

References

Mar 15, 1991·The Biochemical Journal·J A Werkmeister, J A Ramshaw
Dec 31, 1990·Journal of Immunological Methods·J L McKimm-Breschkin
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T HelaakoskiT Pihlajaniemi
Oct 1, 1973·Archives of Biochemistry and Biophysics·J RosenbloomS Jimenez
Jan 1, 1980·Annual Review of Biochemistry·E Adams, L Frank
Jan 1, 1995·Annual Review of Biochemistry·D J Prockop, K I Kivirikko
Dec 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B Brodsky, N K Shah
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·B Brodsky, J A Ramshaw
May 29, 1997·Nature·H W MewesA Zollner

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Citations

Oct 22, 2005·Applied Microbiology and Biotechnology·Julio BáezJames W Polarek
Apr 2, 2008·Journal of Materials Science. Materials in Medicine·John A M RamshawJerome A Werkmeister
Nov 19, 2003·Advanced Drug Delivery Reviews·David OlsenJames Polarek
Apr 15, 2008·Applied and Environmental Microbiology·Anna SteinleAlexander Steinbüchel
Mar 30, 2004·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Chunlin YangJames W Polarek
Nov 8, 2011·Progress in Polymer Science·Sílvia GomesDavid L Kaplan
Jun 10, 2011·Biotechnology and Bioengineering·Catarina I F SilvaMarc W T Werten
Feb 10, 2012·Journal of Biomedical Materials Research. Part a·Falguni PatiPradeep Kumar Das Mohapatra
Aug 6, 2013·Journal of Biomedical Materials Research. Part a·Yong Y PengJohn A M Ramshaw
Sep 25, 2009·Journal of Biomedical Materials Research. Part a·Yong Y PengJerome A Werkmeister
Oct 9, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·John A M Ramshaw
Aug 24, 1999·Yeast·M W WertenF A de Wolf
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Jan 21, 2012·Biomedical Materials·Jerome A Werkmeister, John A M Ramshaw
Sep 10, 2005·Seminars in Cardiothoracic and Vascular Anesthesia·Gerard R ManeckeStuart W Jamieson
Jul 3, 2019·Bioengineering·Evan Davison-KotlerElena García-Gareta
Jan 15, 2009·Journal of Agricultural and Food Chemistry·Cheng ZhangCharles E Glatz

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