PMID: 9634782Apr 1, 1995Paper

Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium

Bio/technology
M ZangJ Reiser

Abstract

The growth-factor prototrophic Chinese hamster ovary (CHO) SSF3 cell line was previously adapted for growth in serum-free media. Here we present a newly designed medium which allows these cells to grow in the absence of any exogenously added growth factors. To investigate the capacity of CHO SSF3 cells for the efficient production of recombinant proteins in protein-free media, expression plasmids containing either a human single chain urokinase-type plasminogen activator (uPA)-encoding cDNA or a humanized immunoglobulin G (IgG) kappa light chain cDNA were introduced by transfection. The tryptophan synthase (trpB) gene of Escherichia coli was used as a dominantly acting selection marker allowing the cells to survive in a medium containing indole in place of tryptophan. Some of the clones obtained exhibited a stable uPA expression over a period of several months under selective conditions and the yields were up to 74 mg of uPA/l in a bioreactor and the productivity was around 40 mg/day per 10(9) cells. The yields of IgG light chains were up to 118 mg/l and the productivity was in the order of 56 mg/day per 10(9) cells in a bioreactor. These results demonstrate the potential of CHO SSF3 cells for the efficient production of recomb...Continue Reading

References

Oct 1, 1991·Current Opinion in Biotechnology·R E Kellems
Dec 1, 1990·Current Opinion in Biotechnology·R J Kaufman
Oct 1, 1992·Current Opinion in Biotechnology·K Furukawa, A Kobata
Aug 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·F J Darfler
Jul 1, 1990·Biologicals : Journal of the International Association of Biological Standardization·F M Wurm
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S C Hartman, R C Mulligan
Jan 1, 1987·Methods in Enzymology·M M Gottesman
Jan 28, 1983·Journal of Immunological Methods·W L ClevelandB F Erlanger
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Oct 1, 1993·Current Opinion in Biotechnology·M E Reff
Oct 1, 1993·Current Opinion in Biotechnology·C E Warren
Apr 5, 1994·Biotechnology and Bioengineering·C E SandstromE T Papoutsakis

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Citations

Aug 24, 1999·Biotechnology and Bioengineering·X MazurM Fussenegger
Sep 5, 2002·Biotechnology and Bioengineering·James E BaileyPhilip S Tsai
Feb 1, 2006·Applied Microbiology and Biotechnology·S A JeffsH Holmes
Nov 13, 2008·Cytotechnology·Ananth ParampalliAnuradha Subramanian
Nov 13, 2008·Cytotechnology·Saeed U Khan, Martin Schröder
Oct 1, 1995·Current Opinion in Biotechnology·J J TrillS Ganguly
Mar 10, 2004·Journal of Biotechnology·Martin SchröderPeter Friedl
Nov 25, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J van der ValkV Baumans
Mar 31, 1999·Trends in Biotechnology·M FusseneggerP P Mueller
Oct 20, 2005·Journal of Bioscience and Bioengineering·Masahiro KawaharaTeruyuki Nagamune
Nov 13, 2008·Cytotechnology·N A SunstromP Gray
Mar 1, 2002·Biotechnology and Bioengineering·Martin SchröderPeter Friedl
Sep 11, 2004·Haemophilia : the Official Journal of the World Federation of Hemophilia·M D TarantinoUNKNOWN RAHF-PFM Clinical Study Group
Jul 11, 2006·Journal of Biotechnology·Claudia AltamiranoFrancesc Gòdia
Jul 11, 2006·Advanced Drug Delivery Reviews·John R Birch, Andrew J Racher

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