Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay

Journal of Microbiological Methods
Hung-Yueh YehRichard J Buhr

Abstract

Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called "Simple Western" was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.

References

Feb 1, 1990·Epidemiology and Infection·H ChartT J Humphrey
Jul 6, 2000·Journal of Clinical Microbiology·F W BrennerB Swaminathan
May 6, 2003·Annual Review of Microbiology·Robert M Macnab
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Jul 24, 2013·Emerging Infectious Diseases·Brendan R JacksonShua J Chai
Sep 21, 2013·Lab Animal·Lisa M Kelly, Leanne C Alworth
Jan 22, 2014·Journal of Medical Microbiology·Hung-Yueh YehBruce S Seal
May 21, 2014·Analytical Biochemistry·John W LoughneyRichard R Rustandi
Jun 3, 2014·Foodborne Pathogens and Disease·Sara M PiresDana Cole
Sep 30, 2014·FEMS Microbiology Letters·Brian B OakleyNelson A Cox
Nov 22, 2014·Archives of Microbiology·Hung-Yueh YehJohn E Line
Dec 5, 2014·PloS One·Jason M Neal-McKinneyMichael E Konkel
Dec 22, 2014·International Journal of Food Microbiology·Marie-Josée J MangenArie H Havelaar
Jan 16, 2015·PLoS Pathogens·Yannick RossezNicola J Holden
Feb 11, 2015·Molecular and Cellular Endocrinology·Viktor Y ButnevGeorge R Bousfield

❮ Previous
Next ❯

Citations

Oct 5, 2017·Expert Review of Proteomics·Manish MishraAldrin V Gomes
Mar 5, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Sophie EdouardMichel Drancourt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.