Production of ribonucleotides by autolysis of Pichia anomala mutant and some physiological activities

Canadian Journal of Microbiology
Jong-Soo LeeCarlos B Miguez

Abstract

Various mutants of Pichia anomala were isolated by ethyl methanesulfonate (EMS) treatment and UV irradiation through cycloheximide resistance and KCl sensitivity. The selected mutant HA-2 accumulated a higher content of RNA and grew faster than the wild-type strain in yeast extract-malt (YM) broth. Autolysis of the HA-2 mutant at 60 degrees C and pH 7.0 for 6 h was the best condition to obtain maximum yields of 5'-ribonucleotides, inosinic monophosphate (IMP) (6.2 mg/g biomass) and guanylic monophosphate (GMP) (35.5 mg/g biomass). The yield of adenylic monophosphate (AMP) (7.8 mg/g biomass) was optimal at 60 degrees C at pH 6.5 for 6 h. The inhibitory activity of the angiotensin-converting enzyme and the nitrite-scavenging activity for autolysates of the HA-2 mutant were about 13.0% and 47.0% higher than those of native strain, respectively.

References

Jul 1, 1971·Biochemical Pharmacology·D W Cushman, H S Cheung
May 1, 1969·Journal of Bacteriology·C T Wehr, L W Parks
Jan 1, 1980·Zeitschrift für allgemeine Mikrobiologie·K I Fayek, S T El-Sayed
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kim Jae-HoLee Jong-Soo

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