PMID: 9177223Jun 10, 1997Paper

Production of secretory immunoglobulin A by a single mammalian cell

Proceedings of the National Academy of Sciences of the United States of America
K R Chintalacharuvu, S L Morrison

Abstract

Secretory IgA (sIgA) plays a critical role in providing protection against infection at the mucosal surfaces. Normally, sIgA is the product of two different cell types with heavy, light, and J chains produced by the plasma cells, whereas secretory component (SC), a cleavage product of the polymeric immunoglobulin receptor (pIgR), is added during the transit of dimeric IgA through the epithelial cell layer. In the current study, by introducing a gene for the processed form of SC into a cell line that produces dimeric IgA, we have succeeded in creating a single cell that is able to produce and secrete covalently joined sIgA. To our knowledge, this is the first time it has been possible to efficiently produce large quantities of sIgA of defined specificity in mammalian cells. The sIgA made using this approach has great potential as an immunotherapeutic.

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Citations

Feb 28, 2002·Journal of Immunological Methods·Esther M YooSherie L Morrison
Jan 30, 2002·Trends in Biotechnology·Blaise Corthésy
May 7, 1999·Immunotechnology : an International Journal of Immunological Engineering·K R Chintalacharuvu, S L Morrison
Sep 22, 2001·Biomolecular Engineering·J W LarrickK Wycoff
May 16, 1998·Molecular Biology of the Cell·K L Singer, K E Mostov
Dec 30, 1999·Biological Chemistry·B Corthésy, F Spertini
Mar 19, 1999·Emerging Infectious Diseases·L ZeitlinK J Whaley
Feb 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Eric W RogierCharlotte S Kaetzel
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BerdozB Corthésy
Oct 30, 2015·Cellular and Molecular Life Sciences : CMLS·Vikram VirdiAnn Depicker
Sep 8, 2010·Biotechnology Advances·Shaista NaqviPaul Christou
Aug 28, 2003·Journal of Plant Physiology·Heribert Warzecha, Hugh S Mason
Jun 1, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Esther M Yoo, Sherie L Morrison
Jul 9, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ruijun ZhangHua-Xin Liao
Apr 6, 2017·Biotechnology Progress·Alexander MatlschweigerRainer Hahn
Apr 4, 2017·Scientific Reports·Katsuhiro NakanishiYasuyuki Imai
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·F E JohansenP Brandtzaeg
Dec 11, 2019·Antibodies·Patrícia de Sousa-Pereira, Jenny M Woof
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G VidarssonJ G van De Winkel
May 20, 2005·Glycobiology·Anton S KarnoupW H Kerr Anderson

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