Production of Sporotrichum thermophile xylanase by solid state fermentation utilizing deoiled Jatropha curcas seed cake and its application in xylooligosachharide synthesis

Bioresource Technology
Ayesha Sadaf, S K Khare

Abstract

De-oiled Jatropha curcas seed cake, a plentiful by-product of biodiesel industry was used as substrate for the production of a useful xylanase from Sporotrichum thermophile in solid state fermentation. Under the optimized conditions, 1025U xylanase/g (deoiled seed cake) was produced. The xylanase exhibited half life of 4h at 45°C and 71.44min at 50°C respectively. It was stable in a broad pH range of 7.0-11.0. Km and Vmax were 12.54mg/ml and 454.5U/ml/min respectively. S. thermophile xylanase is an endoxylanase free of exoxylanase activity, hence advantageous for xylan hydrolysis to produce xylooligosachharides. Hydrolysis of oat spelt xylan by S. thermophile xylanase yielded 73% xylotetraose, 15.4% xylotriose and 10% xylobiose. The S. thermophile endoxylanase thus seem potentially useful in the food industries.

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Citations

Jan 19, 2016·Critical Reviews in Microbiology·Bijender SinghTulasi Satyanarayana
Nov 20, 2015·Bioprocess and Biosystems Engineering·Anju Bala, Bijender Singh
Nov 4, 2015·International Journal of Biological Macromolecules·Uma Shankar Prasad UdayBiswanath Bhunia
Oct 24, 2019·Applied Microbiology and Biotechnology· Alokika, Bijender Singh
Apr 24, 2018·International Journal of Biological Macromolecules·Sunny SharmaBijender Kumar Bajaj

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