PMID: 543803Jan 1, 1979

Production of temperature-sensitive and pathogenic virus from Aedes albopictus cells (Singh) persistently infected with Chikungunya virus

Archives of Virology
A Igarashi


When A. albopictus, clone C6/36, cells were infected with chikungunya (CHIK) virus, high virus yield accompanied by a cytopathic effect in the acute stage of infection was followed by a relatively low yield of virus over a long period of time. Virus produced from persistently infected cultures became gradually of smaller plaque size and more temperature-sensitive; however, such virus still retained pathogenicity for suckling mice even after one year of infection. When the persistently infected cells were subcultured, a dissociation was observed between the time course of cell growth and that of virus production, suggesting some intracellular mechanisms that turn off virus production. The greater part of the interference against CHIK virus by the culture medium of the persistently infected cells appeared to be mediated by the infective virus in the medium. The infective virus was easily removed from the persistently infected cells either by subculture or by cloning in the presence of anti-CHIK serum, yielding cured cultures or virus-negative clones.


Jan 1, 1975·Annals of the New York Academy of Sciences·V StollarR W Schlesinger
Jan 1, 1974·Archiv für die gesamte Virusforschung·J Peleg, V Stollar
Jan 1, 1973·Archiv für die gesamte Virusforschung·P J Enzmann
May 1, 1956·The Journal of Experimental Medicine·T T PUCK, P I MARCUS

Related Concepts

Chikungunya virus
Cytopathogenic Effect, Viral
Neutralization Tests
Viral Interference
Virus Replication
Mouse, Swiss

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