PMID: 9439002Jan 24, 1998Paper

Production of testosterone from cholesterol using a single-step microbial transformation of Mycobacterium sp

Journal of Industrial Microbiology & Biotechnology
W H Liu, C K Lo

Abstract

A novel single-step microbial transformation process for the production of testosterone (TS) from cholesterol by Mycobacterium sp was investigated. It was found that the supply of reducing power, NADH, from the metabolism of glucose was necessary for the reduction of androst-4-en-3,17-dione (AD) to TS. The cultivation time for the maximum accumulation of TS and the residual glucose increased in parallel with the amount of glucose supplemented in fermentation cultures. After the glucose in the fermentation culture was completely consumed, most of the TS was oxidized to AD. Adding a larger amount of glucose could prevent oxidation of TS to AD. Under optimal fermentation conditions, the maximum molar conversion rate of TS from cholesterol was 51% in a 5-L surface-aerated fermentor after 120 h cultivation.

Citations

Feb 13, 1999·Journal of Biotechnology·B Angelova, H P Schmauder
Nov 7, 2000·FEMS Microbiology Letters·L Wadhwa, K E Smith
Aug 23, 2012·Steroids·Haq Nawaz Bhatti, Rasheed Ahmad Khera
Feb 9, 2012·Microbial Biotechnology·J L GarcíaB Galán
Dec 6, 2016·Microbial Biotechnology·Utkarsh SoodRup Lal
Nov 20, 2016·Microbial Biotechnology·Lorena Fernández-CabezónJosé L García
Jul 28, 2019·PloS One·Govinda GuevaraJuana María Navarro Llorens
May 4, 2020·Steroids·Krishika Sambyal, Rahul Vikram Singh

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