Production of the polyclonal antibody against Sudan 3 and Immunoassay of Sudan dyes in food samples

Journal of Agricultural and Food Chemistry
Yong Hua QiJianping Wang

Abstract

In this study, 4-aminophenylacetic acid was covalently coupled to aniline to synthesize an intermediate hapten and the intermediate hapten was coupled to β-naphthol to synthesize a tentative hapten of Sudan 3. The hapten was coupled to bovine serum albumin as the immunogen to produce the polyclonal antibody. The obtained antibody was highly specific to Sudan 3, Sudan 1, and Para red, but showed relative low binding ability to Sudan 2, Sudan 4, and Sudan red G. After evaluation of different coating antigens, a heterologous indirect competitive immunoassay was developed to multidetermine the six red dyes in food samples. The cross reactivities to the six analytes were in a range of 21-105%, and the limits of detection were in a range of 0.1-0.8 ng/mL depending on the compound. Intra- and interassay recoveries from the standard fortified blank samples were in a range of 74.5-96.3% with coefficients of variation lower than 15.1%.

References

Jan 5, 2002·Fresenius' Journal of Analytical Chemistry·M FránekJ Emnéus
Oct 16, 2007·Applied and Environmental Microbiology·Haiyan XuHuizhong Chen
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Laura AnfossiGianfranco Giraudi
Jun 5, 2010·Analytical Biochemistry·Ting XuJi Li

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Citations

Nov 12, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Juan LiHong-Min Liu
Feb 9, 2013·Journal of Agricultural and Food Chemistry·Claude SchummerGilbert Moris
Oct 30, 2016·Critical Reviews in Food Science and Nutrition·Michalina Oplatowska-Stachowiak, Christopher T Elliott
Mar 9, 2018·Analytical and Bioanalytical Chemistry·Wolfgang SchwackGertrud Morlock

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