Production of the subtilase AB5 cytotoxin by Shiga toxin-negative Escherichia coli.

Journal of Clinical Microbiology
Rosangela TozzoliStefano Morabito

Abstract

The subtilase cytotoxin (SubAB) is an AB(5) toxin described in certain Shiga toxin (Stx)-producing Escherichia coli (STEC) strains that usually lack the locus for enterocyte effacement (LEE). We report for the first time the production of SubAB by two Stx-negative E. coli strains, isolated from unrelated cases of childhood diarrhea. The characterization of the SubAB-coding genes showed a 90% nucleotide sequence similarity with that of the prototype subAB, located on the virulence plasmid of the STEC O113 strain 98NK2 (pO113). In both strains, subAB was physically associated with tia, an invasion genetic determinant of enterotoxigenic E. coli. The strains were negative for the saa gene, encoding an adhesin located on pO113 and present in many of the SubAB-positive strains described so far. PCR screening of 61 STEC and 100 Stx-negative E. coli strains in our collection revealed the presence of subAB in five LEE-negative STEC strains but not in the Stx-negative strains. subAB was contiguous to tia in three of the positive strains, which were all negative for saa. These results indicate that SubAB production is not restricted to STEC and suggest that a subAB-tia putative pathogenicity island is involved in the dissemination of subA...Continue Reading

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Aug 6, 2005·Journal of Clinical Microbiology·Rosangela TozzoliStefano Morabito
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Citations

Jun 12, 2013·Canadian Journal of Microbiology·Maria das Graças C PereiraE Robert Atwill
Sep 3, 2010·Nihon saikingaku zasshi. Japanese journal of bacteriology·Kinnosuke Yahiro
Aug 4, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G BuvensD Piérard
Jan 15, 2011·Microbial Pathogenesis·Takeshi FurukawaMasatoshi Noda
Jan 7, 2017·Toxicon : Official Journal of the International Society on Toxinology·E Abril SeyahianElsa Zotta
Sep 28, 2010·Toxins·Adrienne W Paton, James C Paton
Mar 27, 2018·Zoonoses and Public Health·A M SansoN L Padola
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
Sep 4, 2014·Infection and Immunity·Kinnosuke YahiroMasatoshi Noda
Jan 4, 2020·Veterinary Record Open·Magdalena Nüesch-InderbinenRoger Stephan

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