Production of the third and fourth component of complement (C3, C4) by smooth muscle cells

Immunology
Y UedaY Niho

Abstract

Production of the third and fourth components of complement (C3, C4) by smooth muscle cells was investigated by using normal human aortic smooth muscle cells (AoSMC), human smooth muscle cell line (G402) and vascular smooth muscle cells obtained from human umbilical cord vein (UVSMC). AoSMC spontaneously produced both C3 and C4 at 15 ng/10(6) cells/72 hr and 22 ng/10(6) cells/72 hr, respectively, and both were enhanced by interferon-gamma (IFN-gamma). Although phorbol 12-myristate 13-acetate (PMA) and tumour necrosis factor-alpha (TNF-alpha) enhanced C3 production, C4 production was reduced by these agents. On the other hand, G402 produced C4 but not C3 in a dose-dependent manner when cultured with IFN-gamma. UVSMC produced only a small amount of C3 and C4 compared with AoSMC or G402. C3 and C4 produced by AoSMC were confirmed to be identical with their human serum counterparts as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and measurement of haemolytic activity. Northern blotting analysis showed that the expression of mRNA of C3 and C4 was enhanced by TNF-alpha and IFN-gamma, respectively, in AoSMC. Our findings suggest the importance of smooth muscle cells as a source of components of complement i...Continue Reading

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Citations

Apr 8, 2011·American Journal of Respiratory and Critical Care Medicine·Malgorzata WygreckaPhilipp Markart
Oct 11, 2008·The Journal of Clinical Hypertension·Eli MagenMenachem Schlesinger
Mar 23, 2005·Biochemical and Biophysical Research Communications·Hiroshi TsukamotoMine Harada
May 9, 2016·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·John ChapinJeffrey Laurence
May 28, 2005·The American Journal of Pathology·Dana Carina BaiuMichael Noel Hart
Aug 15, 2006·Molecular Immunology·Bo NilssonJohn D Lambris
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Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jameel M InalJürg-A Schifferli
Jul 24, 2018·The American Journal of Pathology·Sri N BatchuVyacheslav A Korshunov

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