Production of thermostable protease enzyme in wastewater sludge using thermophilic bacterial strains isolated from sludge

Water Science and Technology : a Journal of the International Association on Water Pollution Research
J P ChenelR Y Surampalli

Abstract

The volume of sludge produced annually is very high and poses serious disposal problems. The traditional methods of sludge disposal produce secondary pollutants. Therefore, the alternate or suitable solution is reuse of sludge in an ecofriendly approach. Biotechnology is an interesting tool to add value to the processes involved in wastewater and wastewater sludge disposal/reuse. In this context, a study was carried out on thermophilic bacterial strains that produce thermostable proteases. The bacterial strains were first isolated from municipal wastewater sludge. In contrast to the conventional strains used in industries, like Bacillus sp., the new strains were Gram-Negative type. In semi-synthetic medium, a maximal protease activity of 5.25 IU/ml (International Unit per ml) was obtained at a pH of 8.2 and at a temperature of 60 degrees C, which is higher than the stability temperature of 37 degrees C for a similar protease obtained from the conventional producer Bacillus licheniformis. Moreover, growth and protease activity of the strains were tested in wastewater sludge. It is expected that the complexity of sludge could stimulate/enhance the protease production and their characteristics. In conclusion, reuse of wastewater s...Continue Reading

Citations

May 24, 2012·Bioprocess and Biosystems Engineering·James DiakKevin J Kennedy
Sep 17, 2008·Applied Biochemistry and Biotechnology·Faouzi Ben RebahLassaad Belbahri
Apr 11, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Jasper O HardestyCurtis W Frank

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