Production of transgenic chimeric rabbits and transmission of the transgene through the germline

Molecular Reproduction and Development
Szilárd BodóZsuzsanna Bosze

Abstract

Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real-time PCR and was found to be in the range of 0.1-42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras.

References

Aug 11, 1991·Nucleic Acids Research·P W LairdA Berns
Dec 1, 1988·Gamete Research·X Z Yang, R H Foote
Jul 12, 1974·Nature·R L Gardner, A J Munro
Dec 1, 1993·Molecular Reproduction and Development·K H Graves, R W Moreadith
Aug 11, 1993·Nucleic Acids Research·L G LeeW Bloch
Oct 14, 1998·Trends in Genetics : TIG·J Rossant, A Spence
Jan 11, 2000·The Journal of Dairy Research·L HiripiZ Bösze
Mar 30, 2002·Nature Biotechnology·Patrick ChesnéJean-Paul Renard
May 15, 2002·Cloning and Stem Cells·Junichi MatsudaTomoyuki Tokunaga
Jul 27, 2002·Journal of Biotechnology·Louis-Marie Houdebine
Oct 11, 2002·Nature·I WilmutL E Young
Jul 30, 2003·Transgenic Research·Ed J GallagherJim McWhir
Sep 1, 1960·Experimental Cell Research·P S MOORHEADD A HUNGERFORD
Jun 3, 1961·Nature·A K TARKOWSKI

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Citations

Aug 29, 2012·Theriogenology·Z TancosA Dinnyes
Sep 14, 2014·Transgenic Research·Diana JiEdward J Weinstein

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