Production of tumor necrosis factor-alpha by alveolar macrophages of lung cancer patients

Japanese Journal of Cancer Research : Gann
A OkuboT Ogura

Abstract

The abilities of human alveolar macrophages (AM) obtained from healthy donors and patients with lung cancer to produce tumor necrosis factor (TNF) were compared with those of their blood monocytes after activation with lipopolysaccharide (LPS). TNF activity was assayed by measuring cytotoxicity against actinomycin D-treated L929 cells and TNF was determined quantitatively by sandwich enzyme-linked immunosorbent assay (ELISA) with polyclonal and monoclonal antibodies against TNF-alpha. Unstimulated AM from healthy donors released variable amounts of TNF spontaneously, whereas blood monocytes did not. When treated with LPS for 24 h, AM and monocytes produced TNF dose-dependently, but TNF production by AM was significantly more than that by blood monocytes. This TNF activity was inhibited completely by monoclonal anti-TNF-alpha antibody. Macrophages generated by in vitro maturation of monocytes induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) produced more TNF than freshly isolated monocytes. No difference was found in the abilities of AM from healthy donors and patients with lung cancer to produce TNF after activation stimuli. These observations suggest that human AM may be important in in vivo antitumor defen...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Jan 1, 1986·Biochimica Et Biophysica Acta·S Sone
Dec 1, 1989·American Journal of Respiratory Cell and Molecular Biology·S SoneT Ogura
Sep 1, 1988·The American Review of Respiratory Disease·Y MartinetR G Crystal
Feb 1, 1987·The Journal of Clinical Investigation·C F Nathan
Feb 12, 1987·The New England Journal of Medicine·B Beutler, A Cerami
Jan 1, 1973·The Journal of Pathology·G P Velo, W G Spector
Sep 1, 1974·The Journal of Clinical Investigation·E GoldsteinD Warshauer
Oct 1, 1982·British Journal of Cancer·S SwinburneP Cole

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Citations

Jan 1, 1993·Veterinary Immunology and Immunopathology·L W PaceW H Fales
Oct 30, 1999·Respiratory Medicine·H YanagawaS Sone
Mar 21, 2018·Expert Review of Clinical Immunology·J M Carrascosa, E Del-Alcazar
Mar 18, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M DabrowskaR Chazan
Jun 18, 1998·Annals of the New York Academy of Sciences·I L Campbell
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I L Campbell
Oct 17, 2017·Neurology·Jeffrey M GelfandSiddharama Pawate
Apr 1, 1993·Clinical and Experimental Immunology·J MaedaK Higashino

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