Production of Xanthanases by Paenibacillus spp.: Complete Xanthan Degradation and Possible Applications

Iranian Journal of Biotechnology
Simin AshrafParinaz Ghadam

Abstract

Background: A number of microorganisms and their enzymes have been reported as xanthan depolymerizers. Paenibacillus species are well-known polysaccharide hydrolyzing bacteria. However, Paenibacillus alginolyticus and Paenibacillus sp. XD are the only species in the genus which are now known to degrade xanthan. Objectives: Complete biodegradation of the xanthan exopolysaccharide is a rarely found capability among microorganisms. The aim of this study is to survey xanthanase producing bacteria with an appropriate bioactivity for the biopolymer degradation under different environmental conditions. Materials and Methods: The bacteria were isolated based on viscosity reduction of the xanthan solution. Bacterial isolates were identified using rep-PCR (repetitive element-based genomic fingerprinting) and 16S rDNA sequencing. Xanthanases were identified using rep-PCR (repetitive element-based genomic fingerprinting) and 16S rDNA sequencing. Xanthanases were characterized by measuring their activity at different temperatures, pH values, and NaCl concentrations. Degradation of other polysaccharides and xanthan degradation products were investigated based on the screening plate method and TLC (thin-layer chromatography), respectively. Re...Continue Reading

References

Oct 1, 1990·International Journal of Systematic Bacteriology·H SchlesnerE Stackebrandt
Mar 1, 1995·FEMS Microbiology Letters·L González-CandelasD Ramon
Oct 6, 1998·Applied and Environmental Microbiology·W HashimotoK Murata
May 29, 1999·Applied and Environmental Microbiology·H J RuijssenaarsS Hartmans
May 2, 2001·Applied Microbiology and Biotechnology·H J Ruijssenaars, S Hartmans
Oct 11, 2003·Biotechnology Advances·F García-OchoaE Gómez
Oct 4, 2007·International Journal of Systematic and Evolutionary Microbiology·Jongsik ChunYoung-Woon Lim
Feb 12, 2008·Current Protocols in Molecular Biology·K Wilson
Jul 4, 2015·Enzyme and Microbial Technology·Shailesh S SawantBeom Soo Kim

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Citations

Jul 5, 2021·International Journal of Biological Macromolecules·Tahreem RiazJingjing Chen

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