Production, optimization, purification, characterization, and anti-cancer application of extracellular L-glutaminase produced from the marine bacterial isolate

Preparative Biochemistry & Biotechnology
Hanaa OrabiNagwa Sidkey

Abstract

L-glutaminase from bacterial sources has been proven to be effective and economical agents in cancer therapy, food industry and high-value chemicals like threonine. In the present study, a newly isolated bacterial strain was potentially producing extracellular L-glutaminase, it identified as Bacillus subtilis OHEM11 (MK389501) using the 16S rRNA gene. L-glutaminase production optimized and the optimum factors for production under submerged fermentation were at pH 6.5-7.0 and 35 °C after 28 hr using rhamnose and glutamine as carbon and nitrogen sources, respectively, while bagasse was the best inducer for the production under solid-state fermentation. Ethanol precipitation and ion-exchange chromatography using QFF are the purification steps. L-glutaminase was purified to 2-fold with specific activity 89.78 U/mg and its molecular weight about 54.8 kDa with the alkaline property of the enzyme makes it clear having carcinostatic property; maximum enzyme activity at pH 8.2 and 40 °C and retained about 90% activity for 1 hr. The cytotoxicity effect of L-glutaminase indicated a significant safety on Vero cells with high anticancer activity against NFS-60, HepG-2, and MCF-7 cancer cell lines. The outcomes demonstrated that L-glutaminas...Continue Reading

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Citations

Apr 23, 2020·Current Drug Metabolism·Chandrasai Potla DurthiAnand Kishore Kola
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yasser S MostafaMohamed Hashem
May 27, 2021·Applied Microbiology and Biotechnology·Noora BarzkarMojgan Khodadadi
Jun 12, 2021·Current Opinion in Biotechnology·Jingyu ZhangXueting Liu

Methods Mentioned

BETA
biosensor
PCR
electrophoresis
flow cytometry
light microscopy
ion exchange chromatography
gel filtration

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