PMID: 11313112Apr 21, 2001Paper

Production, purification, and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20)

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
D L LiuL Ma

Abstract

Some Armillariella tabescens (E-20) multienzymes have previously been reported to present detoxifying activities against aflatoxins. In this paper, we describe the isolation purification of an intracellular enzyme, named aflatoxin-detoxifizyme, which exhibited detoxification activity on aflatoxin B(1) (AFB(1)). This aflatoxin-detoxifizyme exhibited a specific activity of 7.09 nmol min/mg at pH 6.0 and 28 degrees C. The apparent molecular mass was 51.8 kDa as determined by SDS-PAGE. The isoelectric point was estimated to be 5.4 and optimum activity for the enzyme was found at pH 6.8 and 35 degrees C. The activity of the purified enzyme was confirmed by Ames test.

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Citations

Jul 21, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·G JardA Lebrihi
Dec 15, 2010·International Journal of Molecular Sciences·Shu GuanCheng Ji
Jun 12, 2009·Drug Metabolism Reviews·Qinghua WuKamil Kuca
Jun 18, 2005·Acta Veterinaria Hungarica·J Varga, Beáta Tóth
Jan 22, 2015·Transgenic Research·Li-zeng GuanQian-yun Xi
Jul 23, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yu FanCheng Ji
Dec 24, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yuan-Zhen WuDa-Ling Liu
May 21, 2016·Frontiers in Microbiology·Ilse VanhoutteLeen De Gelder
Oct 8, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Sahib AlamNaresh Magan
Jun 14, 2016·International Journal of Food Microbiology·Oluwafemi Ayodeji AdeboVuyo Mavumengwana
Aug 20, 2016·International Journal of Food Microbiology·C VerheeckeF Mathieu
Oct 31, 2015·Critical Reviews in Food Science and Nutrition·O A AdeboV Mavumengwana
Oct 25, 2014·Toxins·Lancine SangareYang Liu
Dec 13, 2016·Journal of Dairy Science·Sejeong KimKyoung-Hee Choi
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ilya Lyagin, Elena Efremenko
Mar 6, 2021·Critical Reviews in Food Science and Nutrition·Hongwen XuXiulan Sun
Mar 3, 2021·Food Research International·Yongpeng GuoCheng Ji
Aug 17, 2020·Toxicon : Official Journal of the International Society on Toxinology·Tuncay SöylemezZeki Yıldız

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