PMID: 371Dec 1, 1975

Production, purification, and characterization of an extracellular chitosanase from Streptomyces

Journal of Bacteriology
J S Price, R Storck

Abstract

The synthesis by Streptomyces sp. no. 6 of an extracellular chitosanase was induced by glucosamine. The enzyme was purified to homogeneity by Sephadex G-100, carboxymethyl-cellulose, and diethylaminoethyl-cellulose chromatography. The purified enzyme hydrolyzed chitosan (the beta-1,4-linked polymer of glucosamine) but not chitin nor carboxymethyl-cellulose. The only products of the hydrolysis detectable by paper chromatography were di- and triglucosamine. Sephadex G-100 chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the molecular weight of the enzyme was between 29,000 and 26,000. Acid hydrolysates of the enzyme contained no cysteic acid or glucosamine or other carbohydrate. At 25 C, maximum activity was obtained between pH 4.5 and 6.5. The enzymatic hydrolysis of chitosan occurred over a wide range of temperatures and was maximal at 60 C. The rate of the reaction was inhibited by concentrations of soluble chitosan higher than 0.5 g/liter. The apparent Km calculated from a Lineweaver-Burke plot was 0.688 g/liter at pH 5.5. The enzyme prevented spore germination and caused a significant decrease in the turbidity of germinated spore suspensions of the Mucor strains tested. Such a decre...Continue Reading

Citations

Apr 29, 2006·Applied Microbiology and Biotechnology·Y S LeeY L Choi
Jan 1, 1992·Antonie van Leeuwenhoek·D Jones
Dec 17, 2002·The Journal of General and Applied Microbiology·Crispinus A. OmumasabaKihachiro Ogawa
Sep 29, 2011·Journal of Basic Microbiology·Naosekpam Ajit Singh, Veerubommu Shanmugam
Feb 19, 2010·Bioprocess and Biosystems Engineering·Cristiane Fernandes de AssisEveraldo Silvino dos Santos

Related Concepts

Amino Sugars
Cell Wall
Cell-Free System
Enzyme Induction
Xicil
Exoglycosidases
Hydrogen-Ion Concentration
Hydrolysis
Mucor
Polysaccharides, Bacterial