Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System

Biochemistry Insights
Ke WuTao Li

Abstract

Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD) using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus) instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product.

References

Sep 1, 1993·Anatomia, histologia, embryologia·E Martin, H Rodriguez-Martinez
May 1, 1993·Applied and Environmental Microbiology·M SmithD Goff
Aug 15, 2001·Folia Microbiologica·N Dogra, G N Qazi
Feb 20, 2007·The Journal of Biological Chemistry·Yin-Ru ChiangGeorg Fuchs
May 15, 2007·The Journal of Pharmacology and Experimental Therapeutics·Thierry BordetRebecca M Pruss
Feb 20, 1994·Biotechnology and Bioengineering·A S GhatoraeP J Halling
Jan 19, 2010·Seikagaku. The Journal of Japanese Biochemical Society·Hiroyasu Ogino
May 31, 2011·Applied and Environmental Microbiology·O DrzyzgaJ Perera
Oct 18, 2012·Analytical Biochemistry·Robert J BrownDavid A Ford

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Citations

Sep 29, 2019·International Journal of Molecular Sciences·Hanna BuschUlf Hanefeld
Jan 4, 2019·World Journal of Microbiology & Biotechnology·Victoria GiorgiCarlos García Carnelli

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Methods Mentioned

BETA
light scattering
column chromatography
NMR

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