Production, Purification, and in Vitro Evaluation of the Prebiotic Potential of Arabinoxylooligosaccharides from Brewer's Spent Grain

Journal of Agricultural and Food Chemistry
Belén GómezJose L Alonso

Abstract

Brewer's spent grain (BSG) samples were subjected to a two-step aqueous processing (starch extraction and autohydrolysis) in order to assess their potential as a raw material for obtaining a mixture of arabinoxylooligosaccharides (AXOS) suitable to be use as prebiotics for elderly. After hydrothermal treatment, the liquors were refined by a sequence of purification and conditioning steps including membrane filtration, enzymatic hydrolysis, and ion exchange. The presence of both substituted (degree of polimerization (DP) = 2-10) and unsubstituted (DP = 2-16) oligosaccharides made up of xylose and arabinose (AXOS) were confirmed in purified mixtures (in which total OS content = 84% w/w) by using chromatographic techniques and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Finally, AXOS were evaluated for their prebiotic activity by in vitro fermentation assays using fecal inocula from elderly people, demonstrating that AXOS were slightly better substrates than FOS, in terms of bacterial population shifts as in the production of SCFA.

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Sep 11, 2014·Journal of Agricultural and Food Chemistry·Belén GómezJosé L Alonso

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Citations

Aug 23, 2019·Critical Reviews in Food Science and Nutrition·T Bonifácio-LopesManuela Pintado
Oct 5, 2016·AMB Express·Seema A Belorkar, A K Gupta
Feb 24, 2017·Foods·Theodoros VarzakasFrancis Verpoort
Jul 30, 2019·Journal of Food Biochemistry·Gabriela Vazquez-OlivoJosé Basilio Heredia
Aug 24, 2017·Journal of Food Science·Sana IkramMeng Yin
Dec 13, 2019·Carbohydrate Polymers·Cláudia AmorimLígia R Rodrigues

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