PMID: 45295Jan 1, 1979

Production, purification and properties of a Pichia kluyveri killer toxin

Antonie van Leeuwenhoek
E J MiddelbeekC Stumm


Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8-4.0 and 22--25 degrees C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly. The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19 000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40 degrees C.


Jan 1, 1975·Antonie van Leeuwenhoek·G Philliskirk, T W Young
Mar 1, 1978·Canadian Journal of Microbiology·K Al-Aidroos, H Bussey
Jan 1, 1977·Antonie van Leeuwenhoek·C StummG J de Vries
Jan 1, 1978·Antonie van Leeuwenhoek·T W Young, M Yagiu
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Dec 1, 1972·Antimicrobial Agents and Chemotherapy·A M Jetten, G D Vogels
Jan 19, 1972·Nature: New Biology·H Bussey
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Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS


Jan 1, 1986·Folia Microbiologica·O Bendová
Mar 1, 1980·Antimicrobial Agents and Chemotherapy·E J MiddelbeekH L Muytjens
Jan 1, 1989·Antimicrobial Agents and Chemotherapy·A D SawantD G Ahearn
Dec 31, 2008·Applied and Environmental Microbiology·Jessica De IngeniisIlaria Mannazzu
Dec 1, 2000·Applied and Environmental Microbiology·W B ChenS C Chang
Jun 27, 2001·Applied and Environmental Microbiology·Maurizio Ciani, F Fatichenti
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Jan 1, 1980·Antonie van Leeuwenhoek·E J MiddelbeekG D Vogels
Nov 10, 2012·Fungal Genetics and Biology : FG & B·Antonio SantosDomingo Marquina
Jan 5, 2002·Microbiological Research·Domingo MarquinaJ M Peinado
Apr 1, 1995·FEMS Microbiology Letters·G M WalkerV J Hodgson
Jan 1, 1987·Current Genetics·M el-SherbeiniD J Mitchell
Mar 24, 2017·Toxins·Ignacio BeldaAntonio Santos

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