Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Michael Douglas LaneMavis Agbandje-McKenna

Abstract

Adeno-associated viruses (AAVs) are actively being developed for clinical gene-therapy applications and the efficiencies of the vectors could be significantly improved by a detailed understanding of their viral capsid structures and the structural determinants of their tissue-transduction interactions. AAV8 is approximately 80% identical to the more widely studied AAV2, but its liver-transduction efficiency is significantly greater than that of AAV2 and other serotypes. The production, purification, crystallization and preliminary X-ray crystallographic analysis of AAV8 viral capsids are reported. The crystals diffract X-rays to 3.0 A resolution using synchrotron radiation and belong to the hexagonal space group P6(3)22, with unit-cell parameters a = 257.5, c = 443.5 A. The unit cell contains two viral particles, with ten capsid viral protein monomers per crystallographic asymmetric unit.

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Citations

Aug 30, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xuan ShenMark A Kay
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Michael DiMattiaMavis Agbandje-McKenna
Dec 18, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Odayme QuesadaMavis Agbandje-McKenna
May 18, 2012·Journal of Virology·Brittney L GurdaMavis Agbandje-McKenna
Aug 31, 2007·Journal of Virology·Hyun-Joo NamMavis Agbandje-McKenna
Aug 24, 2012·Journal of Virology·Maxim SalganikRobert McKenna
Sep 27, 2013·Journal of Virology·Vamseedhar RayaproluBrian Bothner
Apr 6, 2011·Journal of Molecular Biology·Britta GerlachBettina Böttcher
Jul 18, 2016·Journal of Virological Methods·Yu-Shan TsengMavis Agbandje-McKenna
Jul 11, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zhijian WuR Jude Samulski
Sep 10, 2015·Microbiology Spectrum·Max SalganikRichard Jude Samulski

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