Production, purification, crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 5

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Michael DiMattiaMavis Agbandje-McKenna

Abstract

Adeno-associated virus serotype 5 (AAV5) is under development for gene-therapy applications for the treatment of cystic fibrosis. To elucidate the structural features of AAV5 that control its enhanced transduction of the apical surface of airway epithelia compared with other AAV serotypes, X-ray crystallographic studies of the viral capsid have been initiated. The production, purification, crystallization and preliminary crystallographic analysis of empty AAV5 viral capsids are reported. The crystals diffract X-rays to beyond 3.2 A resolution using synchrotron radiation and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 264.7, b = 447.9, c = 629.7 A. There is one complete T = 1 viral capsid per asymmetric unit. The orientation and position of the viral capsid in the asymmetric unit have been determined by rotation and translation functions, respectively, and the AAV5 structure determination is in progress.

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Citations

Feb 28, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Fredric P ManfredssonRonald J Mandel
Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Edward B MillerMavis Agbandje-McKenna
Dec 18, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Odayme QuesadaMavis Agbandje-McKenna
Nov 11, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Qing XieMichael S Chapman
Feb 6, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Thomas F LerchMichael S Chapman
Jul 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Matthew MitchellMavis Agbandje-McKenna
Jun 14, 2013·Journal of Virology·Brittney L GurdaMavis Agbandje-McKenna
Sep 24, 2010·Journal of Virology·Robert NgMavis Agbandje-McKenna
Sep 27, 2013·Journal of Virology·Vamseedhar RayaproluBrian Bothner
May 19, 2009·Journal of Virological Methods·Kim Van VlietRichard O Snyder
Jul 11, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zhijian WuR Jude Samulski
Aug 9, 2013·Journal of Virology·Lakshmanan GovindasamyMavis Agbandje-McKenna
Nov 21, 2014·Journal of Virology·Sandra AfioneJohn A Chiorini
May 20, 2018·Journal of Neurodevelopmental Disorders·Erik Allen LykkenSteven James Gray
May 1, 2019·Viruses·Justin J KurianMavis Agbandje-McKenna
Sep 10, 2015·Microbiology Spectrum·Max SalganikRichard Jude Samulski
Jun 4, 2020·Molecular Therapy. Methods & Clinical Development·Marti Cabanes-CreusLeszek Lisowski

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