Production rates of prostaglandin F, 6-keto-PGF1 alpha and thromboxane B2 by perifused human endometrium

Prostaglandins
G C LigginsS J Skinner

Abstract

Human endometrium obtained from fresh hysterectomy specimens was perifused for 7 hr in 95% O2/5% CO2 at 37 degrees C. The phase of the menstrual cycle was determined by histological examination. The concentrations of PGF, 6-keto-PGF1 alpha and TxB2 in 20 min fractions of the perifusion medium were measured by radioimmunoassay and production rates were calculated in terms of dry weight of tissue. Biphasic patterns of production were observed; high initial values fell to about 20% at 2 hr and then increased to relatively stable values at about 4 hr which were maintained for the next 2 hr. During this latter period, production rates in endometria taken at different phases of the cycle differed markedly from each other; the production rates of PGF in secretory and early proliferative endometria were low (15.8 +/- 2.6, mean +/- SEM and 67.2 +/- 8.3 ng/min/g respectively) whereas they were high in late proliferative and premenstrual endometria (188.0 +/- 16.7 and 196.4 +/- 16.9 ng/min/g respectively). The patterns of production of 6-keto-PGF1 alpha and TxB2 were similar to those of PGF but the absolute values were much lower (less than 10%). We conclude that the observed rates of production of prostaglandins by perifused human endome...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A HamF A Kuehl
Nov 3, 1977·Nature·S A SaeedH O Collier
Mar 1, 1978·Prostaglandins·J A Salmon
May 1, 1975·Angewandte Chemie·W Bartmann
Sep 1, 1977·Analytical Biochemistry·F A FitzpatrickF F Sun
Feb 1, 1975·Prostaglandins·E M Cane, C A Villee
Jun 1, 1975·Journal of Steroid Biochemistry·J S RobertsJ A McCracken
May 1, 1976·British Journal of Obstetrics and Gynaecology·E A WillmanS G Clayton
Apr 30, 1971·Annals of the New York Academy of Sciences·P Piper, J Vane
Dec 2, 1967·Nature·A BennettJ R Vane
Jul 1, 1973·Journal of Steroid Biochemistry·M Briggs
Apr 1, 1965·The Journal of Obstetrics and Gynaecology of the British Commonwealth·V R PICKLESG N SMITH
Jan 1, 1974·The Journal of Physiology·J DownieM Wunderlich

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Citations

Feb 1, 1985·Journal of Steroid Biochemistry·L MarkiewiczE Gurpide
Jul 1, 1988·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R NemmeE Papiernik
Dec 1, 1987·Prostaglandins·M J PeekR Markham
Mar 1, 1990·Prostaglandins·A Aitokallio-Tallberg
Mar 31, 1990·Lancet·D J FitzgeraldG A FitzGerald
Apr 1, 1985·Prostaglandins, Leukotrienes, and Medicine·M C Rees, A C Turnbull
Jun 1, 1985·Prostaglandins, Leukotrienes, and Medicine·A JouanenA Crastes de Paulet
Sep 1, 1986·Prostaglandins, Leukotrienes, and Medicine·O IshiharaK Satoh
Jan 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P V Peplow, P R Hurst
Dec 1, 1984·Annals of the Rheumatic Diseases·J M KatzD H Gray
Jun 1, 1985·American Journal of Obstetrics and Gynecology·O Ylikorkala, U M Mäkilä
Jan 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P V Peplow, P R Hurst
Feb 1, 1987·Cancer Letters·A Aitokallio-TallbergO Ylikorkala
Oct 1, 1986·British Journal of Obstetrics and Gynaecology·M C Rees, R W Kelly
Sep 1, 1986·British Journal of Obstetrics and Gynaecology·L Mäkäräinen, O Ylikorkala
Jun 1, 1984·Prostaglandins·S J SkinnerG Neale
Apr 24, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Ramkumar Menon, Stephen J Fortunato
May 1, 1982·British Journal of Obstetrics and Gynaecology·L Mäkäräinen, O Ylikorkala
Apr 1, 1990·Annals of Medicine·L Rönnberg

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