Products of oxidized L-ascorbic acid damage acellular DNA.

The Journal of Nutrition, Health & Aging
W ZhangX Jia

Abstract

L-ascorbic acid can be pro-oxidant and anti-oxidant in different reaction. This study aims to test the effects about products of oxidized L-Ascorbic Acid on acellular DNA. Acellular DNA, nuclear DNA fixed on slides, are used in our experiment. There are four groups and one negative. Negative control is sham-treated with buffer(pH 7.2 and AA/ H2O2/fenton free). Experimental groups are treated separately with 0.06 mM L-ascorbic acid (AA) alone(exposed in air), 0.06 mM L-ascorbic acid (AA) alone(no exposure in air), 1.2 mM hydrogen peroxide (H2O2) alone, and a mixture of final concentration of 0.03 mM L-ascorbic acid and 0. 6 mM hydrogen peroxide (AA+ H2O2). Each experimental group consists of 4 slides and each slide is treated for 4 hours at 4 °C in a dark place. The DNA damage is quantified by alkaline Comet Assay. The comet images are analysed by Comet A1.0 software. Differences among groups are compared with SPSS 11.0. DNA singlestrand breakage is found to be treatment-dependent in the following sequence: AA+ H2O2> AA(oxidized) > H2O2, AA(without oxidization) and Control. Acellular DNA can tolerate the low concentration H2O2, but is sensitive to free radical. The results indicate that AA expose in air and mixture of AA and H2O...Continue Reading

References

Jan 1, 1993·Brain Research. Brain Research Reviews·R A Grünewald
Jul 25, 2000·Journal of Chromatography. a·J C Deutsch
May 2, 2002·Nature Medicine·Matthias A Hediger
Aug 23, 2005·Life Sciences·Ionara Rodrigues SiqueiraCarlos Alexandre Netto
Mar 9, 2006·Journal of Nutritional Science and Vitaminology·Yoko InaiMorimitsu Nishikimi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.