Products of the fos and jun proto-oncogenes bind cooperatively to the AP1 DNA recognition sequence

Environmental Health Perspectives
G RisseR Müller

Abstract

The products of the proto-oncogenes c-fos and c-jun form a tight protein complex that is a major component of the transcription factor AP1. To analyze the role of fos in the binding of this complex to the AP1 DNA recognition sequence and the mechanism of interaction in further detail, we have expressed a fos protein in E. coli using an expression vector containing the temperature-inducible lambda PL promoter and a synthetic translational start codon. The fos protein encoded by this construct (termed Baf) was enriched by biochemical purification techniques and was found to form a specific complex with c-jun obtained by in vitro transcription/translation. As shown in gel retardation assays, the baf/jun complex binds to the AP1 DNA recognition sequence with high affinity, while no significant binding was observed with either of the individual protein components, indicating cooperative DNA binding of the two proteins. The fact that the bacterial baf protein does not undergo glycosylation indicates that the post-translational modification of eukaryotic c-fos with N-acetylglucosamine is not required for the formation of a stable fos/jun/DNA complex.

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Citations

Mar 1, 1997·Brain Research. Molecular Brain Research·R Herbst, M J Nicklin
Mar 18, 2008·Respiratory Research·Krishnaswamy G TirumurugaanMathur S Kannan
Sep 6, 2008·PLoS Computational Biology·R SathyapriyaSaraswathi Vishveshwara
Nov 26, 2008·Journal of Molecular and Cellular Cardiology·Dan HuangKai Huang
Jul 5, 2005·General and Comparative Endocrinology·Enrique ZudaireFrank Cuttitta
Aug 1, 1996·Zygote : the Biology of Gametes and Early Embryos·B Wu
Oct 1, 1994·Critical Reviews in Oncology/hematology·M Piechaczyk, J M Blanchard

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