Products of the grg (Groucho-related gene) family can dimerize through the amino-terminal Q domain.

The Journal of Biological Chemistry
M Pinto, C G Lobe

Abstract

The murine grg (Groucho-related gene) products are believed to interact with transcription factors and repress transcription, thereby regulating cell proliferation and differentiation. Most proteins in the grg family contain all of the domains found in the Drosophila Groucho protein, including the S/P (Ser-Pro-rich) domain required for interaction with transcription factors and the WD40 domain, which is thought to interact with other proteins. However, at least two Grg proteins contain only the amino-terminal Q (glutamine-rich) domain. We examined whether the Q domain is used for dimerization between Grg proteins, using the yeast two-hybrid system and binding assays with glutathione S-transferase fusion proteins. We found that Grg proteins are able to dimerize through the Q domain and that dimerization requires a core of 50 amino acids. Surprisingly, the dimerization does not require the leucine zipper located within the Q domain.

References

Jan 1, 1992·Genes & Development·G J KatoC V Dang
Feb 21, 1992·Cell·C A KeleherA D Johnson
Jan 1, 1991·Methods in Enzymology·D M Becker, L Guarente
Aug 1, 1994·Trends in Genetics : TIG·S Fields, R Sternglanz
Dec 1, 1994·Genes & Development·K KomachiA D Johnson
Dec 31, 1993·Cell·M E Fortini, S Artavanis-Tsakonas
Jan 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C Leon, C G Lobe

❮ Previous
Next ❯

Citations

Jan 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C Leon, C G Lobe
Oct 21, 1999·Biochimica Et Biophysica Acta·J Roose, H Clevers
May 7, 1998·The International Journal of Biochemistry & Cell Biology·R KageyamaK Tomita
Mar 16, 2005·Nature Structural & Molecular Biology·Danette L Daniels, William I Weis
Apr 30, 2009·The Journal of Biological Chemistry·Rachel Larder, Pamela L Mellon
Sep 23, 2011·The Journal of Biological Chemistry·Nayan J Sarma, Nabeel R Yaseen
Feb 23, 2012·The Journal of Biological Chemistry·Somasish Ghosh DastidarSantosh R D'Mello
Oct 25, 2006·Molecular and Cellular Biology·Thomas HeimbucherThomas Czerny
May 4, 2004·Molecular and Cellular Biology·Haiyun SongAlbert J Courey
Jun 3, 2009·Genome Biology·Michelle Simon, John M Hancock
Aug 3, 2010·PloS One·Brandon Beagle, Gail V W Johnson
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·S X CaoS R Spindler
Jun 6, 2003·Journal of Molecular Biology·María Ana Gómez-Ferrería, Javier Rey-Campos
Nov 24, 2007·EMBO Reports·Barbara H JenningsDavid Ish-Horowicz
Apr 29, 2008·Journal of Neuroscience Research·Xiaoguang ZhangSantosh R D'Mello
Oct 7, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brandon Beagle, Gail V W Johnson
Nov 24, 2004·Biochemical and Biophysical Research Communications·Yuequn WangXiushan Wu
Apr 25, 2000·Developmental Biology·S SugiyamaH Nakamura
Jan 22, 2011·Cancer Cell·Masahiro SonoshitaMakoto Mark Taketo
Jun 15, 2016·Stem Cells International·Shoichiro KokabuEijiro Jimi
Apr 30, 2005·Journal of Cellular Biochemistry·Malgorzata Gasperowicz, Florian Otto
Jan 11, 2018·BMC Biotechnology·Gerlinde DoenzThomas Czerny
Nov 20, 1998·Molecular and Cellular Biology·G ChenA J Courey
Oct 3, 2002·The Journal of Biological Chemistry·Maina Lepourcelet, Ramesh A Shivdasani

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.