Proenkephalin system in human polymorphonuclear cells. Production and release of a novel 1.0-kD peptide derived from synenkephalin

The Journal of Clinical Investigation
O VindrolaV E Nahmod

Abstract

In the hematopoietic system a pluripotent stem cell generates precursors for lymphoid and myeloid lineages. Proenkephalin-derived peptides were previously detected in differentiated lymphoid cells. We have studied whether the proenkephalin system is expressed in a typical differentiated cell of the myeloid lineage, the neutrophil. Human peripheral polymorphonuclear cells contain and release proenkephalin-derived peptides. The opioid portion of proenkephalin (met-enkephalin-containing peptides) was incompletely processed, resulting in the absence of low molecular weight products. The nonopioid synenkephalin (proenkephalin 1-70) molecule was completely processed to a 1.0-kD peptide derived from the COOH-terminal. This molecule was characterized in neutrophils by biochemical and immunocytochemical methods. The chemotactic peptide FMLP and the calcium ionophore A23187 induced the release of the proenkephalin-derived peptides, and this effect was potentiated by cytochalasin B. The materials secreted were similar to those present in the cell, although in the supernatant a higher proportion corresponded to more processed products. The 1.0-kD peptide was detected in human, bovine, and rat neutrophils, but the chromatographic pattern of...Continue Reading

References

Jun 1, 1976·Scandinavian Journal of Immunology·A Bøyum
Jan 1, 1988·Annual Review of Immunology·N E Sibinga, A Goldstein
Feb 15, 1989·Biochemical and Biophysical Research Communications·A AseV Nahmod
Jan 1, 1986·Annual Review of Pharmacology and Toxicology·V Höllt
Jan 1, 1987·Annals of the New York Academy of Sciences·E G Fischer, N E Falke
Aug 3, 1987·Life Sciences·J E MorleyN P Plotnikoff
Jan 1, 1987·Annual Review of Cell Biology·T L Burgess, R B Kelly
Mar 15, 1988·Biochemical and Biophysical Research Communications·K Yoshikawa, T Aizawa
Apr 1, 1988·Brain Research·R FolkessonL Terenius
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R D HowellsS Udenfriend
Dec 8, 1986·Life Sciences·H J MonsteinL Terenius
Nov 1, 1986·The Journal of Endocrinology·J D WatsonC J Hinds
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D L KilpatrickS Udenfriend
Jan 1, 1966·Annual Review of Physiology·C De Duve, R Wattiaux
Sep 1, 1982·The Journal of Clinical Investigation·B DewaldM Baggiolini
Sep 13, 1982·Biochimica Et Biophysica Acta·H B KaplanG Weissmann
Jan 1, 1984·Annual Review of Biochemistry·J DouglassE Herbert
Jun 1, 1983·The Journal of Cell Biology·R GennaroM Baggiolini
Jul 27, 1984·Neuroscience Letters·D Liston, J Rossier

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Citations

Feb 18, 1994·Brain Research. Developmental Brain Research·M I Rodriguez VidaO Vindrola
May 1, 1993·Free Radical Biology & Medicine·N RabgaouiJ Torreilles
Feb 13, 2001·Developmental and Comparative Immunology·M Salzet, A Tasiemski
Oct 25, 2002·Mediators of Inflammation·Zofia SulowskaHenryk Tchórzewski
May 16, 2014·Pharmaceuticals·Guangshun Wang
Jul 17, 2003·Journal of Neuroimmunology·Sarah HookGraham Le Gros
Mar 2, 2005·European Journal of Pain : EJP·H L Rittner, C Stein
Apr 1, 1991·European Journal of Clinical Investigation·J V Weinstock
Oct 25, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Heike L RittnerAlexander Brack
Oct 6, 2006·Current Opinion in Anaesthesiology·Heike L RittnerChristoph Stein
Jun 15, 2020·European Journal of Pharmacology·Yali TuoMing Xiang
Jan 7, 2004·Biochemistry·Nicole C KaneiderChristian J Wiedermann

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