PMID: 40581Jan 1, 1979

Proestrus and metestrus rat uterus, a rapid and simple in vitro method for detecting histamine H2-receptor antagonism

M Eltze


The antagonism caused by metiamide and cimetidine on histamine-induced inhibition of spontaneous and electrically stimulated isometric contractions of superfused rat uterine horns from proestrus and metestrus stages was studied in vitro. Histamine depressed smooth muscle "twitch" responses of spontaneously contracting or electrically stimulated uterine preparations of both stages in the same dose-dependent manner. The typical effects of organ relaxation, inhibition of contraction height and reduction of resting tension generated by histamine, could both be antagonized by the histamine H2-receptor blockers metiamide and cimetidine, while both compounds failed to reverse orciprenaline- or isoproterenol-induced inhibition of uterine contractility. Diphenhydramine, a histamine H1-receptor antagonist, was not able to reduce histamine-induced inhibition of uterine contractions, thereby confirming that the histamine receptors of the rat uterine tissue are H2 in type. Furthermore, beta-adrenergic blockers, like propranolol and dichloroisoproterenol, failed to antagonize the decrease in contraction amplitude but prevented fall in resting tension induced by histamine. Tyramine, cAMP or dibutyryl-cAMP produced no inhibition of motility of...Continue Reading

Related Concepts

Histamine H2 Antagonists
Metiamide Monohydrochloride
Uterine Contraction

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