Proficiency testing for the detection of Middle East respiratory syndrome coronavirus demonstrates global capacity to detect Middle East respiratory syndrome coronavirus.

Journal of Medical Virology
Torsten TheisWilliam D Rawlinson

Abstract

The first reported case of Middle East respiratory syndrome coronavirus (MERS-CoV) infection was identified in Saudi Arabia in September 2012, since which time there have been over 2000 laboratory-confirmed cases, including 750 deaths in 27 countries. Nucleic acid testing (NAT) is the preferred method for the detection of MERS-CoV. A single round of a Proficiency Testing Program (PTP) was used to assess the capability of laboratories globally to accurately detect the presence of MERS-CoV using NAT. A panel of eleven lyophilized specimens containing different viral loads of MERS-CoV, common coronaviruses, and in vitro RNA transcripts was distributed to laboratories in all six World Health Organization regions. A total of 96 laboratories from 79 countries participating in the PTP, with 76 of 96 (79.2%) reporting correct MERS-CoV results for all nine scored specimens. A further 10 laboratories (10.4%) scored correctly in eight of nine specimens of the PTP. The majority of laboratories demonstrated satisfactory performance in detecting the presence of MERS-CoV using NAT. However, some laboratories require improved assay sensitivity, reduced cross contamination of samples, and improved speciation of coronavirus subtypes for potentia...Continue Reading

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Dec 16, 2016·Journal of Clinical Microbiology·Katherine A LauWilliam D Rawlinson

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Citations

Aug 29, 2019·Der Internist·V M CormanM Witzenrath
Mar 1, 2019·BMJ Global Health·Cassandra Kelly-CirinoMaria D Van Kerkhove
May 29, 2020·Journal of Medical Virology·Nevio Cimolai

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Datasets Mentioned

BETA
JX869059.2

Methods Mentioned

BETA
PCR

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