Apr 25, 2019

Profile of HBV Integration in the Plasma DNA of Hepatocellular Carcinoma Patients

Current Genomics
Weiyang LiQingsheng Kong

Abstract

Hepatitis B Viral (HBV) infection is one of the major causes of Hepatocellular Carcinoma (HCC). Mounting evidence had provided that the HBV integration might be a critical con-tributor of HCC carcinogenesis. To explore the profile of HBV integration in the plasma DNA, the method of next-generation sequencing, HBV capture and bioinformatics had been employed to screen for HBV in-tegration sites in the plasma samples. In the initial experiment, a total of 87 breakpoints were detected in the 20 plasma samples. The distribution of breakpoints showed that there was significant enrichment of breakpoints in the region of intron. Furthermore, the HBV breakpoints were prone to occur in the region of X protein (1,700-2,000bp) in the plasma samples. The pathway analysis had revealed that the HBV integrations sites were specifically enriched in the cancer pathway. Altogether, our results had provided direct evidence for the HBV integration in plasma DNA, and they might be potentially useful for future HCC prognosis and diagnosis.

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Mentioned in this Paper

Biochemical Pathway
Liver Carcinoma
Hepatitis B Virus
Bio-Informatics
Site
Massively-Parallel Sequencing
Plasma
Introns
Analysis
DNA

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