Profile of rhBMP-2 release from collagen minipellet and induction of ectopic bone formation

Drug Development and Industrial Pharmacy
Hiroo MaedaKeiji Fujioka

Abstract

For a cylindrical controlled-release formulation using collagen as a carrier, called the minipellet (MP), which contains rhBMP-2, the relationship between the diameter of MPs and rhBMP-2 release profiles was investigated, and its effect in inducing bone formation was evaluated. Samples with three different diameters were tested for each of the following formulations: MP without additives, MP with 10% (w/w) glutamic acid (Glu) and 20% (w/w) alanine (Ala), and MP with 20% (w/w) Glu and 20% (w/w) Ala. The results of the in vitro release test and the amount of rhBMP-2 remaining in the MPs after subcutaneous implantation into mice were compared among different samples. It was found that the addition of Glu accelerated release of rhBMP-2 effectively. Release was accelerated as the diameter of MP became smaller and the amount of Glu added increased. The amount of calcium formed in 3 weeks after subcutaneous implantation into mice was dose-dependent. The amount of calcium formed per unit rhBMP-2 dose tended to increase as the diameter of MP became smaller and the amount of Glu added became greater; calcification was thus associated with release rate. These results indicate that MPs with smaller diameters induce bone formation more effi...Continue Reading

References

Jan 1, 1997·Journal of Biomedical Materials Research·M L Renier, D H Kohn
Nov 25, 1998·Journal of Biomedical Materials Research·K WhangK E Healy
Jun 24, 1999·Journal of Biomedical Materials Research·H UludagJ Wozney
Oct 21, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·M MaedaK Fujioka
Aug 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hiroo MaedaKeiji Fujioka

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Citations

Apr 26, 2014·Journal of Biomedical Materials Research. Part a·Jung-Seok LeeChang-Sung Kim

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