Profilin is associated with transcriptionally active genes.

Nucleus
Emilia SöderbergNeus Visa

Abstract

We have raised antibodies against the profilin of Chironomus tentans to study the location of profilin relative to chromatin and to active genes in salivary gland polytene chromosomes. We show that a fraction of profilin is located in the nucleus, where profilin is highly concentrated in the nucleoplasm and at the nuclear periphery. Moreover, profilin is associated with multiple bands in the polytene chromosomes. By staining salivary glands with propidium iodide, we show that profilin does not co-localize with dense chromatin. Profilin associates instead with protein-coding genes that are transcriptionally active, as revealed by co-localization with hnRNP and snRNP proteins. We have performed experiments of transcription inhibition with actinomycin D and we show that the association of profilin with the chromosomes requires ongoing transcription. However, the interaction of profilin with the gene loci does not depend on RNA. Our results are compatible with profilin regulating actin polymerization in the cell nucleus. However, the association of actin with the polytene chromosomes of C. tentans is sensitive to RNase, whereas the association of profilin is not, and we propose therefore that the chromosomal location of profilin is...Continue Reading

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Citations

Jan 1, 2013·Cellular and Molecular Life Sciences : CMLS·Kei Miyamoto, J B Gurdon
Feb 6, 2016·Histochemistry and Cell Biology·Ildikó KristóPéter Vilmos
Sep 18, 2013·Experimental Cell Research·Kazumasa AoyamaNaoto Yamaguchi
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Sep 5, 2019·Nature Cell Biology·Christopher Patrick CaridiIrene Chiolo
Jul 15, 2020·Biophysics Reviews·Rhonda J Davey, Pierre Dj Moens
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Aug 26, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul B ComishDaolin Tang
Dec 23, 2020·Journal of Biochemistry·Tomas VenitPiergiorgio Percipalle
Dec 6, 2017·Biochemical and Biophysical Research Communications·Xin Xie, Piergiorgio Percipalle

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Methods Mentioned

BETA
immunoprecipitation
confocal microscopy
dissection

Software Mentioned

DNA LaserGene
Protean
LSM

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