Profiling and functional analysis of differentially expressed circular RNAs in high glucose-induced human umbilical vein endothelial cells

FEBS Open Bio
Guoxi JinMinglong Li

Abstract

Dysfunction of vascular endothelial cells often results in diabetic vascular complications. Circular RNAs (circRNAs) have been implicated in the pathogenesis of various diseases, including diabetes and many vascular diseases. This study aimed to explore the roles of circRNAs in high glucose-induced human umbilical vein endothelial cells (HUVECs) to elucidate the contributions of circRNAs to diabetic vascular complications. We subjected control and high glucose-induced HUVECs to RNA sequencing and identified 214 differentially expressed circRNAs (versus control HUVECs, fold change ≥ 2.0, P < 0.05). We then validated seven of these differentially expressed circRNAs by qPCR (hsa_circ_0008360, hsa_circ_0005741, hsa_circ_0003250, hsa_circ_0045462, hsa_circ_0064772, hsa_circ_0007976, and hsa_circ_0005263). A representative circRNA-microRNA (miRNA) network was constructed using the three most up-regulated circRNAs (hsa_circ_0008360, hsa_circ_0000109, and hsa_circ_0002317) and their putative miRNA. Bioinformatic analysis indicated that these circRNAs regulate the expressions of genes involved in vascular endothelial function and angiogenesis through targeting miRNAs. Our work highlights the potential regulatory mechanisms of three cruc...Continue Reading

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Citations

Sep 1, 2020·Current Eye Research·Miao HeWei Wang
Sep 29, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Ling AnNing Zhang
Jan 19, 2020·Microvascular Research·Tian-Rong Zhang, Wei-Qiang Huang
May 26, 2020·Life Sciences·Qidong CaoChunli Song
Mar 13, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hengquan WanShunlin Qu
May 14, 2021·Molecular Therapy. Nucleic Acids·Jianyi LiChengliang Deng

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Methods Mentioned

BETA
PCR

Software Mentioned

miRanda
Statistical Program for the Social Sciences ( spss )
MEM
BWA
CIRI
seqtk
FastQC
edgeR
clusterProfilers
cBot

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